Part:BBa_K653000:Experience

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Applications of BBa_K653000

Experience of the ITB_Indonesia iGEM 2015 team.

The iGEM 2015 ITB Indonesia used this coding sequence to make BBa_K1685011 part. The Biosurfactor was placed under T7lac promoter and RBS (BBa_K613010) with a double terminator (BBa_B0015) for rhamnolipid production in E. coli BL21(DE3). After IPTG induction, the separated supernatant was tested for surfactant activity.
Supernatant from empty cell, either induced or not, had no / little surfactant activity. The uninduced transformant had little activity, but the induced transformant had higher surfactant activity.
This shows that the part was induced for enzyme expression and biosurfactant production. To our knowledge, this is the very first time that the part was used for expression.

User Reviews

UNIQaa49753dbf9b4618-partinfo-00000001-QINU UNIQaa49753dbf9b4618-partinfo-00000002-QINU